April 10, 2012 – 7:25 pm
Volume 11 (2): 257-270. July (2012)
Ultrasonographic Diagnosis of Pancreatic Surgical Affections in Dogs and Cats
Nefissa H. Mekkawy,* Gomaa M.,** Kramer M.,*** Omar M.S.,* and Samy M. T.*
* Department of Surgery, Faculty of Veterinary Medicine, Zagazig University, Egypt.** Research student (Ph.D. Thesis) Department of Surgery, Faculty of Veterinary Medicine, Zagazig University, Egypt. *** Department of Veterinary Clinical Sciences, Small Animal Clinic, Justus- Liebig University, Giessen, Germany.
ABSTRACT
In the present study, a total number of 19 dogs and 9 cats has been examined and diagnosed ultrasonographically with pancreatic surgical affections. In dogs, it was found that the most common encountered cases were acute pancreatitis 11 out of 19 cases (57.9%) and revealed thickened irregular hypoechoic tissue of mixed echogenicity in the pancreas. In the second level were abscess 3 dogs with hypoechoic mass surrounded by hyperechoic area with echogenic irregular wall. The rest sporadic cases were chronic pancreatitis (2 cases), tumor (2 cases), and cyst (1 dog). While in cats acute inflammation (5 cases out of 9 were the common affection while the rest were cysts (2 cases), abscess (1 case) and chronic inflammation (1 case). The interpretation of the sonographic picture, blood analysis, cytological, bacteriological and histopathological findings as well as surgical treatment of these cases were carried out.
Key words: Ultrasonographic, Pancreas, surgical affections, dogs, cats.
March 6, 2012 – 5:53 pm
EVALUATION OF PLURIPOTENCY OF CANINE ADIPOSE DERIVED MESENCHYMAL STEM CELLS AND THEIR EXTENSION PROPERTIES ON POLY LACTIC- CO- GYCOLIC ACID SCAFFOLD
AHMED ISMAIL A1, 3*, MURUA ESCOBAR H1, LANGNER K1, SCHWENKE A2, WILLENBROCK S1, ABD EL-AAL AM3, NOLTE I1, WEFSTAEDT P1, SAMY MT3
1Small Animal Clinic, Foundation University of Veterinary Medicine, Hannover, Germany
2Laser Zentrum Hannover e.V., Hannover, Germany
3Department of Surgery, Anaesthesiology and Radiology, Faculty of Veterinary Medicine, Zagazig University, Egypt
*This work is extracted from the Doctor of Philosophy Thesis of Ahmed Abdelbaset Ahmed Attia Ismail (2011)
ABSTRACT
Adipose derived mesenchymal stem cells (ADMSCs) have been considered as a valuable and an easy accessible source for pluripotent cell. Up till now, the use of well characterized canine ADMSCs (cADMSCs) in favor of veterinary regenerative medicine has been questioned due to lack of proper definition concept regarding pluripotency specific gene expression. Therefore, the present study was carried out to define un-differntiated cultivated cADMSCs by a marker set for gene expression confirming the pluripotent characteristics of these cells, hence relative quantitive RT-PCR (RT – qPCR) and conventional PCR experiments were generated. GPDH mRNA transcript was used as housekeeping gene. Positive gene expression of OCT4, SOX2, KLF4, cMYC and NANOG was confirmed by RT-qPCR and RT-PCR. As well, the evaluation of their affinity to proliferate and extend in vitro on the surface of collagen – coated PLGA scaffold was conducted. Scanning Electron Microscopy (SEM) revealed uniform distribution as well as significant incorporation of ADMSCs on PLGA scaffolds. This study concluded that, PLGA – ADMSCs constructs might be of value and effective in the repair of large bone defects.
March 6, 2012 – 5:39 pm
استخدام طيوف الامتصاص لتحديد نوعية ورم الميلانومين الموضعي والمنتشر
1غصون معراوي، 2 فواز سيوف، 3 محمد العبدالله، 1محمد أنور بطل
1قسم الفيزياء- كلية العلوم- جامعة حلب ، 2 المعهد العالي لليزر- دمشق ، 3 قسم علم الأحياء- كلية العلوم-
جامعة حلب
الخلاصة:
تسهم دراسة طيوف الامتصاص للأنسجة الحيوانية )أورام ميلانومين(، في المعرفة المسبقة عن
كون ورم ما موضعي أم منتشر في النسيج الحيوي، لذلك تم في هذا البحث إثارة أورام سرطانية
)ميلونومية( في جلد هامستر ذهبي سوري وذلك في المنطقة السفلى من الظهر بتطبيق موضعي
لمادة كيميائية مسرطنة ديمثيل بينزأنثراسين مذابة بالأسيتون وممزوجة بزيت معدني ثقيل مرة
واحدة أسبوعيا على التوالي لمدة 11 أسبوع، و تم تشخيص حالة الورم وتحديد مستواه خلال فترة
زمنية محددة. حُقن نسيج ورم الميلانومين الموضعي بمركب جليكاتد شيتوسان بتركيز 1%، والذي
يستخدم كمساعد مناعي في العلاج التطبيقي، ودرس طيف امتصاص ورم الميلانومين الموضعي
المحقون بالمركب السابق وغير المحقون، وكما أجريت مقارنة أطياف الامتصاص للنسيج الطبيعي
مع الورم الموضعي المحقون ثم مع الورم المنتشر المحقون، وذلك ضمن مجال الأطوال
الموجية 400-1000 نانومتر. ولزيادة معامل الامتصاص ضمن ورم الميلانومين الموضعي بهدف
تحسين فعالية المعالجة الليزرية حُقن النسيج الحيواني )منطقة الورم( أيضا بمادة حساسة أو ماصة
للضوء كمركب اندوسيانين غرين، وأجريت مقارنة أطياف الامتصاص للنسيج الطبيعي مع نسيج
الورم الموضعي ثم مع نسيج الورم المنتشر بهدف تحديد نوعية الورم فيما إذا كان موضعي أو
منتشر من خلال دراسة طيف الامتصاص في مجال الأطوال الموجية السابق. كما تم حقن ورم
الميلانوميا الموضعي بتراكيز مختلفة ل اندوسيانين غرين بهدف تحديد التركيز الأفضل للمعالجة.
كلمات مفتاحية: قياس طيف الامتصاص، ورم جلدي، هامستر ذهبي سوري، تشخيص حالة الورم
موضعي، منتشر.
March 6, 2012 – 5:31 pm
A SEASONAL DYNAMICS STUDY OF ZOOPLANKTON CLADOCERA CALMAN IN SABKHAT AL-JABBUL
هيثم كرباج – فهد زروف – محمد الأحمر
قسم الإنتاج الحيواني – كلية الزراعة – جامعة حلب – حلب – سوريا
الملخص
ABSTRACT
The phylum rotifera is considered as the most important plankton in the freshwater ecosystem; together with the order Cladocera, and the class of Copepoda form zooplankton, which named secondary production. No fish fingerlings growth will continue without this natural food and finally it represents (10-90) % from total fish food of pond culture in fish farms
دراسة تصنيفية لأهم العوالق الحيوانية …
March 6, 2012 – 5:25 pm
الفونا السمكية في بحيرة الحمرات (سبخة الجبول) محافظة حلب – سورية
هيثم كرباج* – صياح الشوفي* – ملك عفاشة**
* قسم الإنتاج الحيواني – كلية الزراعة – جامعة حلب – حلب – سوريا
** قسم العلوم الأساسية – كلية الزراعة – جامعة تشرين – اللاذقية – سوريا
الملخص
ABSTRACT
This research includes classification study of Teleoste fish in AL-Hamrat lake (AL-Jabboul Sabkah), in addition to the study of relative stock of fish species in relation to each other. During this study, classification of seven fish species belonging to five genera from three families were carried out. The more common species was Mugil fish (Liza abu) (27%), whereas the less common was barbell fish (Barbus grypus) (3%), and the more common species by weight was also Mugil fish (Liza abu) (25%), and the less common also was barbell fish (Barbus grypus)(7%) from all the catchments all over the year.
Key words: Fish fauna – AL-Hamrat lake – AL-Jabboul Sabkah
March 6, 2012 – 5:23 pm
EFFECTS OF ASCORBIC ACID AND GLUTATHIONE ON IN VITRO MATURATION OF NEW ZEALAND WHITE AND BALADI BLACK RABBIT OOCYTES
DORRA, T.M.*; A.E. ABDEL-KHALEK**; A.H. RAYA*; S.A. FAWZY***; SH. M. SHAMIAH*** AND I, T. EL-RATEL***
ABSTRACT
The main objective of this study was to evaluate the effects of level of some antioxidants; such as ascorbic acid (AA) and reduced glutathione (GSH), on in vitro maturation rate of New Zealand white (NZW) and Baladi black (BB) rabbit oocytes. Oocytes were collected from 20 NZW and 20 BB rabbit. Oocytes were immediately subjected after collection to slicing technique. Only compact cumulus oocytes (COCs) were used in this study. Five types of maturation medium were used in this study included TCM-199 supplemented with 2 or 3 mM of AA and 0.5 or 1 mM of GSH as well as control medium (without supplementation). The medium was initially equilibrated in CO2 incubator (5% CO2, at 38°C with saturated humidity) for 60 minutes, then the oocytes were cultured in the medium under the same conditions for 20 h as a maturation period. Thereafter, oocytes were fixed (with 3 ethanol: 1 glacial acid), stained (with 1% orcein in 45% acetic acid), and microscopically examined. The present results revealed that supplementation with 2 mM AA yielded the highest (P<0.05) percentage of NZW oocytes arrested at M-II stage (70.59%), but did not differ significantly from that achieved with the supplemental level of 0.5 mM GSH (67.27%). Supplementation with 3 mM AA gave significantly higher (P<0.05) percentage (62.50%), and supplementation with 1 mM GSH exhibited significantly similar percentage (57.5%) of NZW oocytes arrested at M-II as compared to that of their respective control medium (58.73 %). As for BB oocytes, supplementation with 3 mM AA yielded the highest (P<0.05) percentage of oocytes arrested at M-II stage (79.39%), but did not differ significantly from that (77.5%) obtained with the low AA supplemental level (2 mM).On the other hand, supplementation with either level of GSH (0.5 and 1 mM) failed to improve percentage of BB oocytes arrested at M-II as compared to that achieved with their respective control medium (58.33 and 64.12 vs. 71.76%, respectively). Overall percentage of oocytes arrested at M-II was higher in BB than that in NZW does (70.10 vs. 62.97%). Conclusively, the present results may suggest that the Effects Of Ascorbic Acid And Glutathione On In Vitro …
supplementation of TCM-199 with AA had a beneficial effect on the in vitro maturation of rabbit oocytes; and that can be achieved at level of 2 mM for NZW and 3 mM for BB oocytes.
Keywords:Rabbit, NZW, Baladi, ascorbic acid, glutathione, in vitro maturation.
January 14, 2012 – 9:04 am
Volume 10, No. 2 (2011) المجلد العاشر العدد الثاني
January 14, 2012 – 8:59 am
Volume 10 No.2 (2011) المجلد العاشر العدد الثاني
Study of some physicochemical properties of coat fibers in camels
Abd ElGhany, W. H.
Wool Production and Technology Department,
Animal and Poultry Division, Desert Research Center, Cairo, Egypt
The present study was done in Sidi-Barrani region (120 km west of Matrouh city of Egypt). Hair representative samples were obtained from the fleeces of 16 adult one – humped local camels (8 males and 8 females) at the end of August, 2009. Hair samples were divided into two types of fibers; outer coarse fibers and inner fine fibers to study and compare the chemical composition of local camel fibers in relation to their physical characteristics. No significant differences were found in amino acids content between male and female hair fibers except the amino acid Proline which showed a marked increase in female fibers than males, while the non significant numerical increase was noticed in the amino acids; Gly, Cys, Leu, His, Lys and Arg which were increased in male hair fibers content than in females, although these values didn’t reach to the significant levels. In terms of fine inner coat fibers, the differences of hair characteristics between groups were not significant, but the differences of IM% and FM% of outer coat between males and females reached to (P < 0.05).Within groups, the differences of hair physical characteristics between outer and inner coat fibers, in both males and females, were significant (P < 0.01) except CM% and IM% fibers in male group. The overall mean of the amino acid; Cys of males was significantly (P<0.05) higher than in females. The fine fibers (under coat) contained higher amounts of Cys than the coarse fibers (outer coat) in both of males and females. The coarse fibers of males and females had more content of the two amino acids; Thr and Glu than the fine fibers. The overall mean of the amino acid; Pro recorded a significantly increase in hair fibers of females, as compared with males. The amino acid Lys was found to be positively and highly correlated (P<0.01) with FD, while the STL was found to be positively correlated (P<0.05) with the amino acid; Cys. The amino acid; Gly was found to be negatively correlated (P<0.05) with the kemp percentage (K %), in male hair fibers.
Keywords: Amino acids composition, Hair fibers, Camels.
January 14, 2012 – 8:56 am
Volume 10 No.2 (2011) المجلد العاشر العدد الثاني
Impact of wells water on survival of small ruminant in shalateen-halaib region, Egypt
YOUNIS, F. E
Animal and Poultry Physiology Department, Animal and Poultry Division, Desert research Center, Mataria, Cairo, Egypt
This study was carried out in Shalateen- Halaib region. Shalateen-Halaib has a vital strategic importance to Egypt, located in the south-eastern of Egypt desert. It is characterized by broad biodiversity of water resources, soil, plant and animal. Animal production industry (raising, trading and marketing) is main source of income for most inhabitants in the region (El-Shaer, 2000). The present work was designed to throw some lights on utilization the wells water and its influence on some physiological response in small ruminant which growing in this area and relation between their contents of heavy metals such as Pb, Fe, Cu, Cd, Mn, Zn and Sr with survival the sheep and goats in this region in dry grazing period (May–November) and green grazing period (December – April). Heavy metals significantly increased the production of TNF-α, IL-1β andIL- 6 while decreasing the production of IL-8. Nonetheless, cytokines may function as biomarkers in elucidating the mechanisms of the immunotoxic effects of heavy metals. Better understanding to maximize the utilization of available local resources; consequently implementing proper measures and approaches to overcome the prevailing constraints are considered of almost importance for increasing productivity of animal in the a vital strategic region.
Keywords: Small ruminant, Water wells, Heavy metals, Cytokines, Adaptation
January 14, 2012 – 8:23 am
المجلد العاشر العدد الثاني ( Volume 10 No.2 (2011
Effect of somatotropin administration on reproductive hormones and some metabolic parameters of cycling Egyptian ewes
*El-Far, F.I; ** El-Nour, Hayat,vH.M. and ** Mahmoud, Mona A
* Physiology, Dep, Fac. Vet. Med., Cairo University
** Biology, Dept, Animal Reproduction Research Institute Al Ahram- Giza, hayatelnour@yahoo.com
The objective of this study was to evaluate the effects of recombinant bovine Somatotropin (rbST) on some reproductive hormones and metabolites related to ovarian activity during luteal phase with two different doses of somatotropin. This study was carried out on fifteen sexually mature local crossbred ewes (Barki ×Rahmani) of 2-3 years old and of average weight 50-60 kg. Estrous cycle was synchronized by two doses of PGF2α (Prosolvin) injection of 1ml of 5 mg into vaginal submucosa 10 days a part. Ewes were randomly allotted into three groups of 5 ewes each, the first was kept as control, the second and the third groups were subcutaneously injected with 2.5 and 5 mg of somatotropin / day respectively, and continued to cover complete estrous cycle. Individual blood samples were collected daily throughout the experimental periods. High dose somatotropin has an over all increament effect on serum progesterone level on comparison with each of control and low dose somatotropin group. Also, both doses of somatotropin have an overall increasing effect on serum estradiol 17β and IGF1 levels. Androstenedione level was lower in treated ewes compared with control. Moreover, High dose of rbST treated group has an overall increment effect on serum glucose and cholesterol levels. High density lipoprotein (HDL) level in high rbST dose group was higher than that of eachother two groups. Both of overall means of low density lipoprotein (LDL) of control and high dose bST groups were higher than that of low dose somatotropin one. All previous parameters were dependent on the day of the luteal phase. We concluded that higher dose of rbST (5mg/day) subcutaneously injection has positive effect on the reproductive hormones as progesterone, estradiol and IGF1. The same effect of high dose rbST was clear on the levels of serum glucose and lipid profiles. So we recommended using 5 mg of rbST injection to cycling ewes to optimize reproductive management and fertility via its increasing effect on reproductive hormones and metabolites which led to enhance follicular growth and ovarian activity.
Key words: ewe rbST sex hormones IGF1 glucose lipid profiles.
